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目的 观察白细胞介素 - 12 (IL- 12 )对重组日本血吸虫谷胱苷肽 - S-转移酶 - 32 k D融合蛋白 (r Sj GST- Sj32 )蛋白免疫小鼠肝脏虫卵肉芽肿大小的影响。方法 BAL B/ c小鼠 36只随机分成 IL- 12加 r Sj GST- Sj32组、r Sj GST- Sj32组和磷酸盐缓冲液 (PBS)组 ,第 3次免疫后 4周 ,经腹部贴片感染 10条尾蚴 ,45天后杀鼠取肝组织 ,常规染色 ,光镜下进行组织学观察及单个虫卵肉芽肿大小的测量。结果 与 PBS组和 r Sj GST- Sj32组相比 ,IL- 12加 r Sj GST- Sj32组虫卵肉芽肿体积明显减少(P<0 .0 1)。在形态学上 ,PBS组和 r Sj GST- Sj32组肝脏虫卵肉芽肿周围有大量嗜酸性粒细胞为主的炎性细胞浸润 ,还有少量的纤维母细胞、类上皮细胞等 ,有的肉芽肿已有纤维形成。IL- 12加 r Sj GST- Sj32组的肝脏虫卵周围仅有少量的嗜酸性粒细胞聚集 ,很少见到胶原纤维。结论 IL- 12能下调 r Sj GST- Sj32蛋白免疫小鼠肝脏虫卵肉芽肿的应答
Objective To investigate the effect of interleukin-12 (IL-12) on the size of liver granuloma in mice immunized with rSj GST-Sj32 protein . Methods Thirty-six BALB / c mice were randomly divided into IL-12 plus rSj GST-Sj32 group, rSj GST-Sj32 group and phosphate buffered saline (PBS) group. Four weeks after the third immunization, Infected 10 cercariae, 45 days after the killing rat liver tissue, routine staining, histological observation under light microscope and a single egg granuloma size measurement. Results Compared with the PBS group and the rSj GST-Sj32 group, the IL-12 plus rSj GST-Sj32 group significantly decreased the size of the ova granuloma (P <0.01). Morphologically, a large number of eosinophilic inflammatory cells infiltrated around the liver granuloma of the PBS group and the r Sj GST-Sj32 group, as well as a small amount of fibroblasts and epithelial-like cells, and some granulation Swollen already has fiber formation. Only a small amount of eosinophils were accumulated around the liver eggs of the IL-12 plus rSj GST-Sj32 group, and collagen fibers were seldom seen. Conclusions IL-12 can downregulate the response of egg granuloma of mice immunized with r Sj GST-Sj32 protein