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目的探寻在骨骼肌去负荷过程中,能够防止Z线改变的对抗措施。方法将大鼠分为对照组(Con)、对照拉伸组(Con+stretch)、悬吊组(TS)和悬吊拉伸组(TS+stretch)4组,对照拉伸组及悬吊拉伸组在对照及悬吊基础上采用石膏固定方法将踝关节固定于背屈位。每组10只大鼠,共计40只。观测比目鱼肌湿重与其肌纤维超微结构的变化。结果与对照组相比,对照拉伸组比目鱼肌重量/体重比明显降低,悬吊组进一步下降;悬吊拉伸组明显高于去负荷组。对照拉伸组比目鱼肌肌纤维超微结构未见明显改变,定量观测表明肌节长度、A带与I带宽度均无显著性改变,肌原纤维与Z线宽度呈降低趋势。TS组Z线呈波浪样改变,肌原纤维与Z线宽度呈显著性缩窄。悬吊拉伸组肌节结构基本正常,肌原纤维与Z线宽度呈降低趋势。结论适当拉伸比目鱼肌可部分阻止去负荷导致的肌肉萎缩,并能防止Z线结构的损伤性改变。
Objective To explore the countermeasures that can prevent the Z line from changing during the process of skeletal muscle de-loading. Methods The rats were divided into four groups: Con group, Con + stretch group, TS group and TS + stretch group, control group and suspension group Extension group in the control and suspension based on the use of gypsum fixation method to fix the ankle joint in dorsiflexion. Each group of 10 rats, a total of 40. Observation of soleus muscle wet weight and its changes of myofibrillar ultrastructure. Results Compared with the control group, the weight / body weight ratio of soleus muscle decreased significantly in the control stretching group and decreased further in the suspension group. The suspension tension group was significantly higher than that in the control group. There was no significant change in the ultrastructure of soleus muscle fibers in the control group. Quantitative observation showed that there was no significant change in sarcomere length, A band and I band width, while the width of myofibril and Z line decreased. TS group Z line showed a wave-like changes, myofibril and Z line width was significantly narrowed. The structure of sarcomere was basically normal in suspension group, and the width of myofibril and Z line showed a decreasing trend. Conclusion Appropriate stretching of the soleus muscle can partially prevent the muscle atrophy caused by the load and prevent the damage of the Z-line structure.