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目的研究苏木素在真菌性角膜炎早期诊断中的价值。方法 2006年6月至2011年6月期间临床诊断为真菌性角膜炎病例59例,角膜刮片,标本分别行苏木素染色、KOH湿片检查和真菌培养。以培养结果做为确诊真菌性角膜炎的“金标准”。结果 59例真菌性角膜炎病例中47例检出真菌,阳性率79.66%。苏木素染色法查找真菌阳性率64.40%,灵敏度74.46%,特异度42.86%,假阳性率25.00%,假阴性率25.53%,正确指数17.32%,阳性预测值92.11%,阴性预测值42.85%。KOH湿片法查找真菌阳性率45.76%,灵敏度44.68%,特异度18.75%,假阳性率50.00%,假阴性率55.32%,正确指数-36.57%,阳性预测值77.78%,阴性预测值18.75%。结论苏木素染色法是真菌性角膜炎早期诊断的快速、有效方法。
Objective To study the value of hematoxylin in the early diagnosis of fungal keratitis. Methods 59 cases of fungal keratitis were diagnosed clinically from June 2006 to June 2011. The corneal scraps were stained with hematoxylin and stained with KOH and examined for fungal culture. To cultivate the results as the diagnosis of fungal keratitis “gold standard ”. Results In 59 cases of fungal keratitis, 47 cases were detected, the positive rate was 79.66%. The positive rate of fungi was 64.40%, the sensitivity was 74.46%, the specificity was 42.86%, the false positive rate was 25.00%, the false negative rate was 25.53%, the correct index was 17.32%, the positive predictive value was 92.11% and the negative predictive value was 42.85%. The positive rate of fungus was 45.76%, the sensitivity was 44.68%, the specificity was 18.75%, the false positive rate was 50.00%, the false negative rate was 55.32%, the correct index was -36.57%, the positive predictive value was 77.78% and the negative predictive value was 18.75%. Conclusion Hematoxylin-eosin staining is a rapid and effective method for the early diagnosis of fungal keratitis.