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Clone20系由S．minnesotaR595死菌免疫Balb／c小鼠制备的IgM类单克隆抗体，曾报告它对脂多糖（LPS）中的末端αKDO（2-酮基-3-脱氧辛酸）特异。本文报告采用ELISA显示，Clone20尚能和LPS的毒性成分类脂A结合。用Clone20免疫的小鼠淋巴细胞制备杂交瘤并筛选出4个分泌抗独特型抗体的细胞株．编码为Clone47－50。ELISA抑制试验显示，单克隆抗体Clone49和Clone50与固相Clone20的结合能为E．coliJ5（Re-型）LPS、S．minnesotaR595（Re-型）LPS、类脂A及αKDO所抑制；Clone47和Clone48则否。另一方面，4个克隆均能抑制Clone20结合到团相Re－LPS及类脂A。在被动溶血试验中，Clone20与固相Re－LPS的结合，也为4个克隆所抑制。对于Clone20与αKDO的结合，仅Clone49与50显示抑制作用。这些结果提示，Clone20分子上与Clones47－50的结合部位均不同程度地接近乃至分享其与类脂A结合的独特型决定簇，Clones49与50则尚可结合Clone20上和。KDO结合的独特型决定簇或其邻近结构。此外，在Clone50的腹水中检出高滴度的抗Re－LPS和抗类脂A抗体。综合以上关于Clone50的免疫学特性，推断它至少是部分模拟了LPS的抗原结构。初步的动物实验显示，提前3hr给小鼠注射Clone50，对随后的致死性内毒素休克有明显对抗作用。这一结果提
Clone20 by S. Minnesota R595 killed BALB / c mice were immunized with IgM monoclonal antibodies that had been reported to be specific for terminal αKDO (2-keto-3-deoxyoctanoic acid) in lipopolysaccharide (LPS). This report reports the use of ELISA to show that Clone20 is still able to bind to lipid A, a toxic component of LPS. Hybridoma was generated from mouse lymphocytes immunized with Clone20 and four cell lines secreting anti-idiotypic antibodies were screened. Code to Clone47-50. ELISA inhibition test showed that the binding capacity of the monoclonal antibodies Clone49 and Clone50 to the solid phase Clone20 is E. coliJ5 (Re-type) LPS, S. minnesotaR595 (Re-type) LPS, lipid A and αKDO inhibited; Clone47 and Clone48 is not. On the other hand, all four clones inhibited the binding of Clone20 to the bulk phase Re-LPS and lipid A. In passive hemolysis assays, the binding of Clone20 to solid-phase Re-LPS was also inhibited by 4 clones. For the binding of Clone20 to αKDO, only Clone49 and 50 showed inhibition. These results suggest that the Clone20 molecule binds Clone47-50 to some extent and even shares its idiotypic determinant with lipid A, while Clones49 and 50 bind Clone20. KDO-bound idiotypic determinants or their adjacent structures. In addition, high titer anti-Re-LPS and anti-lipid A antibodies were detected in ascites of Clone50. Based on the above immunological properties of Clone50, it is inferred that it at least partially mimics the antigenic structure of LPS. Preliminary animal experiments showed that injections of Clone50 3 hours prior to mice had a significant antagonistic effect on subsequent lethal endotoxic shock. This result is mentioned