利用赖氨酸类似物 L—4—氧赖氨酸(OL)作选择剂,通过组织培养,从秋此水稻成熟种胚的愈伤组织中筛选出 L—37、L—38,L—40,L—43抗 OL 突变轴胞系。突变细胞系愈伤组织在不含 OL 培养基上培养10代,对OL 仍有较高的抗性。突变细胞系的淀粉酶、酯酶、过氧化物酶同工酶的活性有所增加,18种氨基酸的含量均有增加,其中赖氨酸含量增加218.8%。L—37愈伤组织酯酶同工酶酶谱在快区前沿多了4条谱带。L—37、L—40得到了再生植株。 L-37, L-38 and L-40 were screened from the callus of mature embryos in autumn by tissue culture using lysine analog L-4-oxyl-lysine (OL) as selective agent , L-43 anti-OL mutant axis cell line. Mutant cell line callus in the absence of OL medium cultured for 10 generations, still have higher resistance to OL. The activity of amylase, esterase and peroxidase isozymes in mutant cell lines increased, the contents of 18 kinds of amino acids increased, and the content of lysine increased by 218.8%. L-37 callus esterase isoenzymes in front of the fast zone more than 4 bands. L-37, L-40 regenerated plants.