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【目的】在体观察缺血再灌注对犬冠状动脉内皮细胞功能的影响以及猪冠状动脉对血管活性药物的反应性。【方法】动脉麻醉后开胸、心包吊床,之后分离冠状动脉左前降支。犬作第一对角支插管,猪作冠状动脉左回旋支插管。犬冠状动脉左前降支缺血30min后再灌注60min,猪不作缺血再灌注。分别向冠状动脉内注射乙酰胆碱和硝酸甘油,以注药后平均冠状动脉血流量变化的百分率来评价冠状动脉内皮细胞依赖性和非依赖性的舒张功能。【结果】注乙酰胆碱后,基线状态下犬平均冠状动脉血流量从(17±4)mL/min升至(41±12)mL/min(P<0.05);但缺血再灌注后舒血管反应幅度降低,其中再灌注15min时间点平均冠状动脉血流量增幅水平明显低于基线增幅水平(142%±54%vs87%±57%,P<0.05)。注乙酰胆碱后,基线状态下猪平均冠状动脉血流量在(11±3)s内从(21±11)mL/min迅速降至(12±8)mL/min,在(42±10)s内又回升至(23±13)mL/min。硝酸甘油使基线状态下犬平均冠状动脉血流量从(18±5)mL/min升至(40±22)mL/min(P<0.05),猪的冠状动脉也呈类似反应。【结论】乙酰胆碱引起犬冠状动脉呈舒血管反应,缺血再灌注使犬冠状动脉内皮细胞功能明显受损。猪冠状动脉对乙酰胆碱呈先收缩后舒张的“双向反应”。硝酸甘油则引起犬或猪的冠状动脉均呈舒张反?
【Objective】 The objective of this study was to observe the effect of ischemia-reperfusion on the function of canine coronary endothelial cells and the reactivity of porcine coronary arteries to vasoactive drugs. 【Methods】 After thoracotomy and pericardial halation, the left anterior descending coronary artery was isolated. Dogs as the first diagonal branch tube, pigs for coronary artery left circumflex artery. Canine left anterior descending artery ischemia 30min after reperfusion 60min, pigs without ischemia and reperfusion. Acetylcholine and nitroglycerin were injected into the coronary arteries, respectively, to evaluate coronary endothelial cell-dependent and independent diastolic function as a percentage of changes in mean coronary blood flow after injection. 【Results】 The average coronary blood flow increased from (17 ± 4) mL / min to (41 ± 12) mL / min in dogs at baseline after acetylcholine injection (P <0.05) The mean coronary artery blood flow increase was significantly lower than baseline at 15 min after reperfusion (142% ± 54% vs 87% ± 57%, P <0.05). After acetylcholine injection, mean coronary blood flow decreased rapidly from (21 ± 11) mL / min to (12 ± 8) mL / min within (11 ± 3) s at baseline and within (42 ± 10) s Again rose to (23 ± 13) mL / min. Nitroglycerin increased mean coronary blood flow at baseline from (18 ± 5) mL / min to (40 ± 22) mL / min in dogs (P <0.05), and similar coronary responses in pigs. 【Conclusion】 Acetylcholine causes a vasodilatory response to canine coronary arteries. Ischemia-reperfusion can significantly impair the function of canine coronary endothelial cells. Porcine coronary artery of acetylcholine was first systolic and diastolic “two-way response.” Nitroglycerin is caused by dogs or pigs showed coronary diastolic reverse?