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目的比较三七总皂甙(PNGS)及维拉帕米(Ver)对大鼠肝脏低温保存的效果和机制。方法采用大鼠离体肝脏再灌注模型(IPRL),用Fura-2法测定低温保存2h后肝细胞内钙离子浓度;经乳酸林格氏液(LR)低温保存24h的肝脏再灌注30min后进行肝脏功能检测、氧自由基代谢产物、能量物质和胆汁流量测定及形态学观察,乳酸林格氏液(LR)和DMEM液中加入不同浓度PNGS和Ver20mg。结果大鼠肝细胞内钙离子浓度、MDA、SOD、AST、ALT、LDH、ATP、TAN、AEC及胆汁流量等项指标各实验组明显好于对照组(P<0.01),PNGS对大鼠肝脏的保护作用显示出剂量依赖性,在200～600mg范围内随剂量增加保护作用随之增强(P<0.01),在800～1000mg范围内虽有增强,但无明显差别(P>0.05)。维拉帕米20mg对大鼠肝脏的保护作用仅同PNGS200～400mg时的作用相当(P>0.05)。结论PNGS对大鼠肝脏低温保存的保护作用总体上优于Ver20mg,估计与其抑制钙超载和抗氧自由基损伤机制不同有关。
Objective To compare the effect and mechanism of panax notoginseng saponins (PNGS) and verapamil (Ver) on rat liver cryopreservation. Methods Rat hepatic reperfusion model (IPRL) was used. The intracellular calcium concentration was determined by Fura-2 method after 2 hours of hypothermia. The liver was stored at 30 ℃ for 24 hours after Lactate Ringer’s solution (LR) Liver function test, oxygen free radical metabolites, energy substances and bile flow measurement and morphological observation, lactate Ringer’s solution (LR) and DMEM solution were added different concentrations of PNGS and Ver20mg. Results The levels of intracellular calcium, MDA, SOD, AST, ALT, LDH, ATP, TAN, AEC and bile flux in the experimental group were significantly higher than those in the control group (P <0.01) (P <0.01) in the range of 200-600 mg, while there was no significant difference in the range of 800-1000 mg (P> 0.05) .Conclusion: The protective effect is dose-dependent. The protective effect of verapamil 20mg on rat liver was only equivalent to that of PNGS 200 ~ 400mg (P> 0.05). Conclusion The protective effect of PNGS on rat liver cryopreservation is generally better than that of Ver20mg, which is estimated to be related to its mechanism of inhibiting calcium overload and antioxidant free radical damage.