川续断(Dipsacus asper)的干燥根以热甲醇回流(3次,每次6h)提取,回流液真空蒸发后,将残渣悬浮在水中,依次用氯仿、乙酸乙酯和正丁醇提取。正丁醇可溶组分上硅胶柱,用氯仿-甲醇-水(52:28:8的下层)洗脱,得到dipsacus皂甙(1)和(2)的混合物。将混合物上硅胶柱,以 The dried roots of Dipsacus asper were extracted with hot methanol reflux (3 times, 6 h each). After evaporation of the reflux liquid in vacuo, the residue was suspended in water and extracted with chloroform, ethyl acetate and n-butanol in that order. The n-butanol soluble fraction was applied to a silica gel column and eluted with chloroform-methanol-water (lower 52:28:8) to give a mixture of dipsacus saponins (1) and (2). Put the mixture on a silica gel column to