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目的探讨发育性髋关节脱位(DDH)和雌激素(ER)基因多态性之间的相关性。方法取2003年2月~2004年8月我院治疗DDH患儿90例,设为DDH组,对照组50例。分别提取DDH组与正常儿童DNA,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法研究ER基因限制性内切酶XbaⅠ和PvuⅡ酶切多态性与DDH发生的关系。结果DDH组和对照组雌激素受体PvuⅡ内切酶酶切多态性分布差异存在显著性意义(P<0.05);DDH组和对照组雌激素受体XbaⅠ内切酶酶切多态性分布差异无显著性意义(P>0.05)。结论雌激素受体PvuⅡ内切酶酶切基因型多态性与DDH的发生之间存在相关性,雌激素受体XbaⅠ内切酶酶切基因型多态性与DDH的发生之间无显著相关性。
Objective To explore the relationship between developmental dislocation of the hip (DDH) and estrogen (ER) gene polymorphisms. Methods From February 2003 to August 2004, 90 children with DDH were treated in our hospital, which were divided into DDH group and control group. The DNA of DDH group and normal children were extracted respectively and the relationship between the restriction endonuclease Xba Ⅰ and Pvu Ⅱ restriction enzyme digestion and the occurrence of DDH was studied by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results There were significant differences in the polymorphism distribution of estrogen receptor PvuⅡ endonucleases between DDH group and control group (P <0.05). The distribution of estrogen receptor XbaⅠ endonuclease digestion in DDH group and control group There was no significant difference (P> 0.05). Conclusions There is a correlation between genotypes of PvuII endonuclease digesting estrogen receptor and occurrence of DDH. There is no significant correlation between polymorphism of XbaI endonuclease digesting estrogen receptor and occurrence of DDH Sex.