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目的:观察RAD16-Ⅱ的物理特性以及神经干细胞在其上黏附和增殖的情况,探讨其构建的细胞假体在脊髓损伤中可能的应用。方法:将RAD16-Ⅱ的饱和水溶液同与之等体积的1.8%的氯化钠溶液混合后静置24h,通过肉眼、光镜和扫描电镜观察其表面结构,并对其吸水性和溶液pH值的动态变化进行评价;将经鉴定的神经干细胞与其共同培养,扫描电镜下观察细胞在材料上的黏附情况,同时应用免疫组化方法评价神经干细胞在材料上的增殖和分化情况。结果:RAD16-Ⅱ在1.8%的氯化钠溶液中塑型后,肉眼和光镜下形态多变,其形状取决于塑型的容器形状;扫描电镜下其为规则的网状,网孔直径为54.00±3.24“m,纤维直径为9.00±1.57”m,网孔底部相互沟通,神经干细胞可在其上迁移和增殖。结论:RAD16-Ⅱ具有良好的物理特性,神经干细胞可在与其构建的假体中迁移和良好地增殖、分化,在脊髓损伤的修复中可能具有潜在的应用前景。
OBJECTIVE: To observe the physical characteristics of RAD16-Ⅱ and the adhesion and proliferation of neural stem cells on it, and to explore the possible application of the constructed cell prosthesis in spinal cord injury. Methods: The saturated aqueous solution of RAD16-Ⅱ was mixed with an equal volume of 1.8% sodium chloride solution and allowed to stand for 24 hours. The surface structure of RAD16-Ⅱ was observed by naked eye, light microscope and scanning electron microscope. The water absorption and pH value The identified neural stem cells were co-cultured with the cells. The adhesion of the cells to the materials was observed under a scanning electron microscope. Meanwhile, the proliferation and differentiation of neural stem cells in the materials were evaluated by immunohistochemistry. Results: The morphologies of RAD16-Ⅱ in 1.8% sodium chloride solution were variable under the naked eye and under the light microscope. The shape of RAD16-Ⅱ was determined by the shape of the plastic container. Under the scanning electron microscope, 54.00 ± 3.24 “m, fiber diameter of 9.00 ± 1.57 ” m, the bottom of the mesh communicate with each other, neural stem cells can migrate and proliferate thereon. CONCLUSION: RAD16-Ⅱ has good physical properties. Neural stem cells can migrate and proliferate well in the prosthesis with which they are constructed, and may have potential application in the repair of spinal cord injury.