论文部分内容阅读
目的:测定转化生长因子β(TGF-β)诱导分子βig-h3 mRNA在人肝癌细胞系中的表达情况。方法:培养人肝癌细胞T7721(转染并稳定高表达HAb18G/CD147)、7721(未转染HAb18G/CD147)、人肝细胞癌细胞系(HHCC)和正常人张氏肝细胞QZG,提取总RNA,设计合成特异性引物,以β-肌动蛋白(β-actin)作为内参照,检测βig-h3 mRNA的差异表达情况。结果:逆转录聚合酶链反应法(RT-PCR)结果显示,TGF-β诱导分子βig-h3的mRNA在人肝癌细胞中的表达水平明显高于正常肝细胞(P<0.01),其在人肝癌细胞系中的表达水平依次为HHCC>T7721>7721,在正常肝细胞QZG中表达水平最低。结论:证明了βig-h3 mRNA在人肝癌细胞系中高表达,为进一步探讨βig-h3在肝癌细胞黏附和转移过程中的作用机制奠定了基础。
Objective: To determine the expression of transforming growth factor β (TGF-β)-induced βig-h3 mRNA in human hepatoma cell lines. METHODS: Human hepatoma cell line T7721 (transfected and stably expressing HAb18G/CD147), 7721 (untransfected HAb18G/CD147), human hepatocellular carcinoma cell line (HHCC) and normal human Zhang cell QZG were cultured and total RNA was extracted. , Design and synthesis of specific primers, β-actin (β-actin) as an internal reference, detection of βig-h3 mRNA differential expression. RESULTS: Reverse transcription-polymerase chain reaction (RT-PCR) results showed that the expression level of TGF-β-induced βig-h3 mRNA in human hepatoma cells was significantly higher than that in normal hepatocytes (P<0.01). The expression level in hepatocellular carcinoma cell lines was HHCC>T7721>7721 in order, and the expression level was lowest in normal hepatocytes QZG. Conclusion: The high expression of βig-h3 mRNA in human hepatocellular carcinoma cell line was established, which laid the foundation for further study of the mechanism of βig-h3 in the process of hepatocellular carcinoma cell adhesion and metastasis.