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应用ＬＳＡＢ免疫组化方法对５２例肝细胞癌（ＨＣＣ）及其肝内转移灶、癌栓中ｎｍ２３Ｈ１和Ｐ５３蛋白表达进行前瞻性研究，探讨两者表达与ＨＣＣ浸润转移及预后的相互关系。结果表明：在原发癌、转移癌和癌栓中，ｎｍ２３Ｈ１和Ｐ５３表达呈显著负相关。原发癌较肝内转移灶和癌栓，ｎｍ２３Ｈ１显著高表达（Ｐ＜０．００２）；而Ｐ５３表达无差异（Ｐ＞０．５）。Ｐ５３在分化较差和包膜浸润的癌组织呈高表达（Ｐ＜０．０２５，Ｐ＝０．０２７）。ｎｍ２３Ｈ１，高表达的Ｈｃｃ患者术后复发率较低（Ｐ＝０．０３６）．Ｐ５３高表达者术后复发率较高（Ｐ＝０．０４８）。证实ｎｍ２３Ｈ１高表达，Ｐ５３低表达的ＨＣＣ患者肝内转移率较低，预后较好。提示ｎｍ２３Ｈ１和Ｐ５３基因及其蛋白的相互或协同作用，是ＨＣＣ浸润转移的重要分子调控机制之一。
LSAB immunohistochemistry was used to prospectively study the expression of nm23H1 and P53 protein in 52 cases of hepatocellular carcinoma (HCC), intrahepatic metastasis, and tumor thrombus, and the relationship between the expression of both proteins and invasion, metastasis and prognosis of HCC was explored. The results showed that there was a significant negative correlation between the expression of nm23H1 and P53 in primary, metastatic and tumor thrombi. The expression of nm23H1 was significantly higher in primary cancer than in intrahepatic metastases and tumor thrombus (P<0.002); however, there was no difference in P53 expression (P>0.5). P53 was highly expressed in poorly differentiated and infiltrated cancer tissues (P<0.025, P=0.027). The postoperative recurrence rate was lower in patients with nm23H1 and high expression of Hcc (P=0.036). Patients with high expression of P53 had a higher postoperative recurrence rate (P=0.048). It was confirmed that the high expression of nm23H1 and the low expression of P53 in HCC patients had lower intrahepatic metastasis rate and better prognosis. It suggests that the interaction or synergistic effect of nm23H1 and P53 genes and their proteins is one of the important molecular regulation mechanisms of HCC invasion and metastasis.