目的　观察体外培养肾小管上皮细胞缺氧、氧损伤后细胞粘附性及α3整合素的改变 ,为细胞粘附性改变在急性缺血性肾功能衰竭发病机制中的作用提供理论依据。方法　采用化学缺氧与氧损伤模型 ,免疫荧光激光共聚焦显微镜观察培养人肾小管上皮细胞α3整合素分子表达及分布的改变 ,微管吸吮法观察细胞与基质以及细胞间粘附力的改变。结果　单纯氧损伤后肾小管上皮细胞α3整合素的表达无显著改变 ,缺氧损伤后其极性分布减弱 ,缺氧 氧损伤后极性分布改变显著 ,这种分布改变与细胞和基质间的粘附性减弱和细胞间粘附性增强显著相关。结论　缺氧、氧损伤后肾小管上皮细胞α3整合素的极性分布改变对肾小管与基质和细胞间的粘附性均有显著影响 ,这种改变有可能在急性缺血性肾功能衰竭中发挥作用 OBJECTIVE: To observe the changes of cell adhesion and α3 integrin in cultured renal tubular epithelial cells after hypoxia and oxygen injury and provide a theoretical basis for the role of cell adhesion changes in the pathogenesis of acute ischemic renal failure. Methods The model of chemical hypoxia and oxygen damage was established. The expression and distribution of α3 integrin in cultured human renal tubular epithelial cells were observed by immunofluorescence laser confocal microscopy. The changes of cell-matrix and intercellular adhesion were observed by microtubule sucking method. Results The expression of α3 integrin in renal tubular epithelial cells did not change significantly after oxygen injury alone. The polarity distribution of renal tubular epithelial cells after hypoxia injury was weakened, and the polarity distribution changed significantly after hypoxia injury. The distribution changed with the adhesion between cells and matrix Discrepancies were significantly associated with increased intercellular adhesion. Conclusions The changes of the polarity distribution of α3 integrin in renal tubular epithelial cells after hypoxia and oxygen injury have a significant effect on the adhesion of renal tubules to stroma and cells. This alteration may play an important role in acute ischemic renal failure Play a role