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目的探讨脑胶质瘤组织中p27~(KIP1)蛋白和S期激酶相关蛋白2(skp2)异常表达与临床病理因素的关系。方法应用免疫组化SP法检测45例胶质瘤和10例正常脑组织中p27~(KIP1)和Skp2蛋白表达情况。结果p27~(KIP1)和Skp2蛋白在正常脑组织和胶质瘤组织中的阳性表达率差异有显著性(P=0.033、P=0.005);p27~(KIP1)和Skp2蛋白表达水平与胶质瘤组织学分级相关(P<0.01),但与患者的性别、年龄及肿瘤大小无明显关系(P>0.05);Skp2阳性表达组和阴性表达组之间p27~(KIP1)蛋白阳性率差异有显著性(x~2=4.8458,P=0.028);胶质瘤组织中p27~(KIP1)蛋白和Skp2蛋白的表达呈负相关(r=-0.5477,P<0.05)。结论Skp2蛋白在胶质瘤组织中表达上调,加速了对p27~(KIP1)泛素化依赖的蛋白降解,使p27~(KIP1)蛋白表达降低,失去对细胞周期的调控并促进细胞异常增殖,从而参与了胶质瘤的发生和发展。
Objective To investigate the relationship between the abnormal expression of p27 KIP1 and skp2 in gliomas and the clinicopathological factors. Methods Immunohistochemical SP method was used to detect the expression of p27 KIP1 and Skp2 protein in 45 gliomas and 10 normal brain tissues. Results The positive expression rate of p27 (KIP1) and Skp2 in normal brain tissue and glioma tissue was significantly different (P = 0.033, P = 0.005). The expression of p27 KIP1 and Skp2 protein was positively correlated with glial (P <0.01), but not with the gender, age and tumor size (P> 0.05). The positive rate of p27 KIP1 protein between Skp2 positive group and negative group was The expression of p27 KIP1 protein and Skp2 protein in glioma tissues was negatively correlated (r = -0.5477, P <0.05). Conclusion The up-regulation of Skp2 protein in glioma tissue accelerates the degradation of p27 KIP1-dependent protein, reduces the expression of p27 KIP1 protein, loses the regulation of cell cycle and promotes the abnormal proliferation of cells. Thus involved in the occurrence and development of glioma.