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研究弓形虫功能性抗原，为开发弓形虫病诊断试剂及疫苗提供理论基础。方法：采用十二烷基硫 酸钠－聚丙烯酰胺凝胶电泳（ＳＤＳ－ＰＡＧＥ）和免疫印迹方法，对弓形虫抗原及弓形虫速殖子抗原中能与免疫兔血清和 弓形虫抗体阳性人血清起特异性反应的抗原组分进行分析。结果：弓形虫速殖子抗原的蛋白组分在ＳＤＳ－ＰＡＧＥ中 用１２５ ｇ／Ｌ的分离胶可产生较好的分辨率，分离出 ３０多条区带。免疫印迹分析表明 ２种抗血清均能特异地识别弓 形虫速殖子抗原，共同识别的抗原组分相对分子质量为 ４３ ０００、３３ ０００、２７ ０００、１４ ０００。结论：这些抗原组分是诱导 宿主产生对弓形虫感染免疫应答的功能性抗原。
Study Toxoplasma gondii functional antigen, to provide a theoretical basis for the development of diagnostic reagents and vaccines. Methods: Toxoplasma gondii antigens and Toxoplasma gondii antigens were detected by SDS-PAGE and immunoblotting in order to compare with those of immunized rabbit serum and Toxoplasma gondii antibody positive human serum Antigen components that specifically react were analyzed. Results: The protein component of tachyzoites of Toxoplasma gondii had better resolution in SDS-PAGE with 125 g / L separation gel, and more than 30 bands were isolated. Immunoblot analysis showed that both antisera could specifically recognize Toxoplasma gondii antigens, and the relative molecular mass of the identified antigen components were 43 000, 33 000, 27 000, 14 000. Conclusion: These antigen components are functional antigens that induce the host to produce an immune response to T. gondii infection.