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利用聚合酶链反应（ＰＣＲ）技术检测了３２例结核性渗出性胸膜炎和２０例非结核性疾病患者胸液中结核杆菌的６５ＫＤａ抗原蛋白编码基因的特异ＤＮＡ片段。结果表明，其检出率为７８．１３％，并具高度特异性。提示ＰＣＲ扩增ＤＮＡ技术对结核性渗出性胸膜炎是高度敏感和特异的早期、快速诊断方法。
The specific DNA fragments of 65KDa antigen protein coding genes of Mycobacterium tuberculosis in pleural fluid of 32 cases of tuberculous exudative pleurisy and 20 cases of non-tuberculous diseases were detected by polymerase chain reaction (PCR). The results showed that the detection rate was 78.13%, and highly specific. Tip PCR amplification of DNA technology for tuberculous exudative pleurisy is highly sensitive and specific early, rapid diagnostic method.