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从肝素和 ACD 抗凝血中分离的淋巴细胞,用 DMSO 或甘油作为低温保护剂,保存在液氮内,在冻前和冻后10、20、40和365天时分别检测细胞的功能活性。当冻存液内含10%DMSO 和40%人 AB 血清时,冻存效果最好。冻存一年时,淋巴细胞的回收率和存活率均大于92.5%。冻存细胞的 E 和 ME 花环形成率、ANAE 阳性率、LDH 同工酶谱和细胞超微结构与冻前相比,均无明显变化,冻存细胞的淋转能力虽在冻存初期有所下降,以后就处于稳定状态。
Lymphocytes isolated from heparin and ACD anticoagulants were treated with DMSO or glycerol as cryoprotectants and stored in liquid nitrogen. Cell viability was measured before and 10, 20, 40 and 365 days after freezing, respectively. Cryopreservation works best when the cryopreservation solution contains 10% DMSO and 40% human AB serum. When frozen for one year, the recovery rate and survival rate of lymphocytes were greater than 92.5%. There were no significant changes in the formation rate of E and ME rosette, ANAE positive rate, LDH isozymes and cell ultrastructure in frozen-thawed cells Decline, after a steady state.