论文部分内容阅读
真菌病毒在真菌中广泛存在,一些真菌病毒可以影响病原真菌营养生长、产孢和色素形成等性状。本文研究了真菌病毒在水稻稻瘟菌中的多样性以及其对寄主生物学性状的影响。采用单孢分离的方法从发病水稻叶片上分离获得了120个稻瘟菌菌株,其中约52%携带dsRNA片段。菌株QSP5被2个基因组为dsRNA的病毒即产黄青霉病毒1-C(Magnaporthe oryzae chrysovirus 1-C,MoCV1-C)和稻瘟菌病毒3(M.oryzae virus 3,MoV3)所侵染。对菌株QSP5进行单个的分生孢子纯化,获得了仅携带MoV3的菌株QSP5-9。菌株QSP5与QSP5-9的产孢量、菌丝形态以及菌落形态存在明显差异,经研究推测MoCV1-C与菌株QSP5的产孢能力衰退、菌丝生长和产色素异常相关。同时对MoV3的稳定性进行了初步的研究。从稻瘟菌菌株QSP5获得了100个单孢分离物,通过对随机挑选的40个单孢分离物进行检测,结果表明这些分离物100%携带MoV3,只有部分单孢分离物携带MoCV1-C。另外,从稻瘟菌菌株QSP5-9获得了94个单孢分离物,对随机挑选的45个单孢分离物进行检测,结果表明这些分离物中均含有与菌株QSP5-9大小相同的dsRNA片段。根据以上结果推定MoV3在稻瘟菌菌株QSP5和QSP5-9及其无性后代中具有一定的稳定性。
Fungal viruses are widespread in fungi and some fungal viruses can affect pathogenic fungal vegetative growth, sporulation and pigment formation. In this paper, the diversity of fungal viruses in Magnaporthe grisea and their effects on host biological traits were studied. A total of 120 M. grisea isolates were isolated from diseased rice leaves using single-spore isolation, of which about 52% carried dsRNA fragments. Strain QSP5 was infected with two viruses genome-wide dsRNA, namely Magnaporthe oryzae chrysovirus 1-C (MoCV1-C) and M. oryzae virus 3 (MoV3). Single conidia purification of strain QSP5 resulted in strain QSP5-9 carrying only MoV3. The sporulation amount, mycelial morphology and colony morphology of the strains QSP5 and QSP5-9 were significantly different. It was speculated that the sporulation ability of MoCV1-C was negatively correlated with the mycelial growth and hypochromism of QSP5. At the same time, the stability of MoV3 has been preliminary studied. One hundred isolates were obtained from the strain of Magnaporthe grisea QSP5, and 40 isolates of spore isolates were randomly selected. The results showed that these isolates contained 100% MoV3 and only some of the isolates had MoCV1-C. In addition, 94 single spore isolates were obtained from Magnaporthe grisea strain QSP5-9 and 45 single spore isolates randomly selected. These isolates contained dsRNA fragments of the same size as strain QSP5-9 . Based on the above results, it is presumed that MoV3 has certain stability in the strains QSP5 and QSP5-9 and their clones.