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目的:研究肝癌细胞系的基因表达概况,筛选表达差异的抑制细胞凋亡相关基因,探索肝细胞癌发生发展机制及肝细胞癌治疗的新靶点。方法:通过基因芯片技术检测肝癌细胞系Bel-7402、Hep-3B与胎儿肝细胞的全基因组序列,筛选表达差异的抑制细胞凋亡相关基因,采用RT-PCR、免疫组化对部分差异表达基因进行了验证。结果:在检测的54614个探针组中,Bel-7402、Hep-3B两株肝癌细胞与胎儿肝脏细胞相比表达共同上调2倍及2倍以上的有1452个基因;表达下调2倍及2倍以上的有2054个基因。与抑制细胞凋亡相关表达上调的基因有12个,选取2个基因进行RT-PCR、免疫组化验证,结果与基因芯片结论基本一致。结论:肝细胞癌中有众多的抑制细胞凋亡基因表达增高,通过影响TNF通路、bcl-2蛋白等多种方式共同抑制细胞凋亡,促进肿瘤细胞增殖。对这些基因的研究有助于阐明肝细胞癌发生发展机制,为肝细胞癌的治疗提供新的靶点。
Objective: To study gene expression profiles of hepatocellular carcinoma cell lines, screen differentially expressed genes that inhibit apoptosis, and explore the mechanisms of hepatocellular carcinogenesis and development and new targets for the treatment of hepatocellular carcinoma. METHODS: The whole genome sequence of hepatocellular carcinoma cell line Bel-7402, Hep-3B and fetal liver cells was detected by gene chip technology. The differentially expressed genes that inhibited apoptosis were screened. RT-PCR and immunohistochemistry were used to detect differentially expressed genes. Verified. Results: In the 54614 probes detected, Bel-7402, Hep-3B liver cancer cells and fetal liver cells expressed 2452 times and more than 1452 genes; the expression was down-regulated by 2 and 2 There are 2054 genes more than doubled. There were 12 genes that were up-regulated by inhibition of apoptosis, and 2 genes were selected for RT-PCR and immunohistochemistry. The results were basically consistent with those of the gene chip. Conclusion: There are many inhibitory apoptotic gene expressions in hepatocellular carcinoma. The expression of apoptotic genes in hepatocellular carcinoma is increased, and apoptosis is promoted by influencing the TNF pathway and bcl-2 protein, which promotes the proliferation of tumor cells. The study of these genes will help elucidate the mechanism of hepatocellular carcinoma development and provide a new target for the treatment of hepatocellular carcinoma.