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目的观察阪崎杆菌侵袭人脑血管内皮细胞后,细胞骨架中微丝和微管的变化。方法用荧光染色技术观察阪崎杆菌侵袭人脑微血管内皮细胞后,细胞骨架中的微丝和微管的变化,观察微丝和微管的抑制剂对阪崎杆菌侵袭人脑微血管内皮细胞的影响。结果阪崎杆菌侵袭人脑微血管内皮细胞后,细胞骨架结构发生明显的改变,微丝和微管部分断裂,模糊,散在分布,微丝抑制剂(细胞松弛素D)和微管抑制剂(秋水仙素)可以抑制阪崎杆菌侵袭人脑微血管内皮细胞。结论阪崎杆菌侵袭人脑血管内皮细胞破坏细胞骨架结构,微丝和微管抑制剂抑制阪崎杆菌侵袭。
Objective To observe the changes of microfilaments and microtubules in the cytoskeleton after inactivation of Saccharomyces saurisq in human cerebrovascular endothelial cells. Methods Fluorescent staining was used to observe the changes of microfilaments and microtubules in the cytoskeleton induced by A. sakazakii invasion of human brain microvascular endothelial cells. The effects of fibrillar and microtubule inhibitors on invasiveness of A. capillaries to human brain microvascular endothelial cells were observed . Results After Enterobacter sakazakii invasion of human brain microvascular endothelial cells, the cytoskeleton structure was changed obviously. The microfilaments and microtubules were partially broken, blurred and scattered, and the microtubule inhibitors (Cytochalasin D) and microtubule inhibitors Narcissus) can inhibit Enterobacter sakazakii invasion of human brain microvascular endothelial cells. Conclusion Inhalation of sakazakii in human cerebrovascular endothelial cells undermines the cytoskeleton structure. Microfilaments and microtubule inhibitors inhibit the invasion of sakazakii.