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建立了辣椒制品中酸性橙Ⅱ的超高效液相色谱-串联质谱分析方法。样品用乙腈提取,WAX弱阴离子固相萃取柱净化浓缩,经超高效液相色谱分离,三重四极杆质谱电喷雾电离(ESI),负离子多反应监测模式检测。结果表明,在1~200ng/mL质量浓度范围内,线性相关系数大于0.998;加标回收率为83.2%~113.2%,相对标准偏差为0.7%~7.2%。检出限分别为1.0μg/kg(辣椒油及辣椒酱)和2.0μg/kg(辣椒粉)。方法适用于辣椒制品中酸性橙Ⅱ的测定。
A method for the determination of acid orange Ⅱ in pepper by ultra performance liquid chromatography-tandem mass spectrometry was established. The samples were extracted with acetonitrile, purified by WAX weak anion solid phase extraction column, separated by ultra performance liquid chromatography, triple quadrupole electrospray ionization (ESI) and negative ion multi-reaction monitoring. The results showed that the linear correlation coefficient was greater than 0.998 in the range of 1 ~ 200 ng / mL, the recoveries were 83.2% -113.2%, and the relative standard deviations were 0.7% -7.2%. The limits of detection were 1.0 μg / kg (chili oil and chili sauce) and 2.0 μg / kg (chili powder) respectively. The method is suitable for the determination of acid orange Ⅱ in pepper products.