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为了了解反义ｃ－ ｍｙｃ 基因在平滑肌细胞中持续、稳定表达对细胞凋亡的影响，将分别载有ｃ－ ｍｙｃ 第１ 、第２ 和第３ 外显子反义片段的三种重组逆转录病毒表达载体ａＭ１、ａＭ２ 和ａＭ３ ，导入体外培养的大鼠主动脉平滑肌细胞，并于持续筛选１ 个月后进行稳定表达检测。结果表明，ａＭ３ 的导入和稳定表达使平滑肌细胞的凋亡率达到对照组的３ ．６ 倍，并伴有明显的细胞体积缩小，而ａＭ１ 和ａＭ２ 的凋亡诱导作用则明显弱于ａＭ３。另外，在超微结构的改变上，转染了ａＭ３ 和ａＭ１ 的平滑肌细胞出现凋亡和自噬的改变，而转染了ａＭ２ 的平滑肌细胞则普遍出现明显的肌丝束。提示，反义ｃ－ ｍｙｃ 的稳定表达促进平滑肌细胞的凋亡，而ａＭ３ 载体在体外促使平滑肌细胞发生凋亡和自噬的能力最强。
In order to understand the effect of continuous and stable expression of antisense c-myc gene on apoptosis in smooth muscle cells, three recombinant reverse transcripts containing antisense fragments of c-myc 1st, 2nd and 3rd exons respectively The virus expression vectors aM1, aM2 and aM3 were introduced into rat aortic smooth muscle cells cultured in vitro and stably expressed for 1 month after continuous screening. The results showed that aM3 import and stable expression of the smooth muscle cell apoptosis rate reached 3 in the control group. 6-fold, accompanied by a significant reduction in cell size, while aM1 and aM2 apoptosis induction was significantly weaker than aM3. In addition, aM3 and aM1 transfected aM3 and aM1 transfected cells showed changes of apoptosis and autophagy, while aM2-transfected smooth muscle cells showed obvious myofibers. It is suggested that the stable expression of antisense c-myc promotes the apoptosis of smooth muscle cells, while the aM3 vector has the strongest ability to induce smooth muscle cell apoptosis and autophagy in vitro.