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目的:探讨中药补肾调经方对提高IVF-ET超排卵周期所获卵和胚胎质量的作用及其机制。方法:将70例因输卵管因素行IVF-ET的患者随机分为中药+控制性超排卵(实验组,n=35)和单纯控制性超排卵组(对照组,n=35),应用Western blotting法测定取卵日成熟卵泡卵泡液中骨形成蛋白-15(bone morphogenetic protein-15,BMP-15)的含量;用RT-PCR法检测颗粒细胞中BMP-15 mRNA的表达;并比较组间的获卵数、受精率、优质胚胎率及妊娠率等。结果:实验组卵泡液中BMP-15mRNA水平及颗粒细胞中BMP-15 mRNA的表达均显著高于对照组(P<0.05);实验组的临床妊娠率(71.42%)显著高于对照组(48.57%)(P<0.05)。结论:补肾调经方可以提高IVF-ET妊娠率,其作用机制可能与调控卵泡液和颗粒细胞BMP-15水平有关。
Objective: To investigate the effect and mechanism of Bushen Tiaojing Recipe (TCM) for improving ovulation and embryo quality obtained from IVF-ET superovulation. Methods: Seventy patients with IVF-ET due to tubal factors were randomly divided into traditional Chinese medicine + controlled superovulation (experimental group, n = 35) and simple controlled ovarian hyperstimulation group (control group, n = 35) Method was used to determine the content of bone morphogenetic protein-15 (BMP-15) in mature follicular fluid of ovum. RT-PCR was used to detect the expression of BMP-15 mRNA in granulosa cells. Obtain the number of eggs, fertilization rate, high-quality embryos and pregnancy rates. Results: The levels of BMP-15 mRNA and BMP-15 mRNA in granulosa cells in the experimental group were significantly higher than those in the control group (P <0.05). The clinical pregnancy rate (71.42%) in the experimental group was significantly higher than that in the control group (48.57 %) (P <0.05). Conclusion: Bushen Tiaojing Recipe can increase the pregnancy rate of IVF-ET, and its mechanism may be related to the regulation of follicular fluid and granulosa cells BMP-15 levels.