目的建立大鼠微小病变型肾病综合征模型,了解低分子右旋糖酐联合速尿对水肿状态下肾病综合征肾小球基底膜阴离子位点是否会产生影响。方法采用连续式静脉输液法给药。标本用透射电镜观察,采用聚乙烯亚胺染色法比较正常组、模型组、盐水组(生理盐水+速尿)与右糖组(低分子右旋糖酐+速尿)肾小球基底膜阴离子位点的变化。结果正常组肾小球基底膜阴离子位点分布规则、间距相等、颗粒较大、数目无缺失;其他各组阴离子位点分布不规则、间距不等、颗粒大小不等、数目均有缺失,按缺失程度由重至轻依次为模型组、盐水组、右糖组,肾小球基底膜内、外疏松层阴离子位点数目在这3组间均有显著统计学差异(P<0.01),而在右糖组与正常组之间则无明显差异(P>0.05)。结论低分子右旋糖酐加速尿应用于水肿状态的肾病综合征,具有促进肾小球基底膜阴离子位点数目快速恢复的作用。 Objective To establish a model of minimal nephrotic syndrome in rats and to understand whether low molecular dextran combined with furosemide affect the glomerular basement membrane anion sites in nephrotic syndrome in edema state. Methods Continuous intravenous infusion method. Specimens were observed by transmission electron microscopy, polyethyleneimine staining was used to compare the anionic sites of glomerulus basement membrane in normal group, model group, saline group (saline + furosemide) and right sugar group (low molecular dextran + furosemide) Variety. Results In the normal group, the distribution of anion sites in glomerular basement membrane was regular, with the same spacing and larger number of particles, with no deletion in number. The anion sites in other groups were distributed irregularly with different spacing and particle size. The number of anions in the model group, saline group, right sugar group, glomerular basement membrane and out of the loose layer were significantly different from each other in all three groups (P <0.01) There was no significant difference between right sugar group and normal group (P> 0.05). Conclusion Low molecular dextran accelerated urine application in edematous nephrotic syndrome, with the promotion of glomerular basement membrane anion site rapid recovery role.