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目的了解该地区部分HIV-1感染者抗病毒治疗前CD4~+ T淋巴细胞、病毒载量分布及耐药性毒株存在情况。方法利用流式细胞技术对CD4~+ T淋巴细胞计数,使用NASBA方法测定病毒载量,利用RT-PCR获得目的基因序列,登陆http://hivdb.stanford.edu分析耐药突变位点。结果该地区感染人群中66.66%的患者CD4~+ T淋巴细胞数>350个/mm~3,患者病毒载量对数平均值为3.784±1.048,检测样本中有2例出现耐药性。结论该地区HIV-1感染者目前多为无症状期,但病毒拷贝数较高,耐药性毒株流行水平较低。应在该地区加强抗病毒治疗的宣传。
Objective To understand the prevalence of CD4 ~ + T lymphocytes, viral load distribution and drug resistant strains before HIV treatment in some HIV-1 infected patients in this area. Methods Flow cytometry was used to count CD4 ~ + T lymphocytes. The viral load was determined by NASBA method. The target gene sequence was obtained by RT-PCR. The site of resistance mutation was analyzed at http://hivdb.stanford.edu. Results The number of CD4 + T lymphocytes was> 350 cells / mm ~ 3 in 66.66% of the infected population in the region, and the average viral load in patients was 3.784 ± 1.048. Two of the tested samples showed drug resistance. Conclusion Most of HIV-1 infected people in this area are mostly asymptomatic, but the virus copy number is higher and the prevalence of drug-resistant strains is lower. Antiviral treatment should be promoted in the area.