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目的:探讨内镜规范清洗消毒对阻止乙肝病毒传播的影响及其重要性。方法:选择在我院进行胃镜检查,且检测前无乙肝病史的病人为研究对象,镜检系统经规范清洗消毒后进行胃镜检测,分别于胃镜前及四次胃镜后取血,采用双抗体一步夹心法酶联免疫吸附试验(ELISA)检测患者血清中HBsAg的含量及用PCR技术检测乙肝病毒标志物HBsAg的DNA表达情况。结果:胃镜前1人测出有HBsAg,数值为0.12;1次胃镜检查组有2人测出有HBsAg,分别为0.169和0.143;2次胃镜检查组有2人测出有HBsAg,分别为0.157和0.125;3次胃镜检查组和4次胃镜检查组均未测出有HBsAg;所有样品均不呈S型曲线,即0次、1次、2次、3次、4次胃镜检查组患者取样均未见乙型肝炎病毒DNA。结论:内镜规范化清洗消毒可有效防止乙肝病毒的传播。
Objective: To investigate the effect of endoscopic cleaning and disinfection on the prevention of hepatitis B virus transmission and its importance. Methods: We selected gastroscopy in our hospital and the patients without history of hepatitis B before the test were selected as the research objects. After the system was cleaned and disinfected by the endoscopy, the gastroscopy was performed. The blood samples were collected before gastroscopy and after gastroscopy four times respectively. Sandwich enzyme-linked immunosorbent assay (ELISA) detection of serum HBsAg levels and detection of hepatitis B virus markers by HBsAg DNA expression. Results: One person before gastroscopy detected HBsAg with a value of 0.12; HBsAg was found in 2 of the 1 gastroscopy group, 0.169 and 0.143, respectively; 2 of the 2 gastroscopy groups had HBsAg, 0.157 And 0.125 respectively. No HBsAg was detected in the third gastroscopy group and the fourth gastroscopy group. All samples showed no S-curve, that is, patients in 0, 1, 2, 3 and 4 gastroscopy groups were sampled No hepatitis B virus DNA was found. Conclusion: Endoscopic cleaning and disinfection can effectively prevent the spread of hepatitis B virus.