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目的建立女运动员三联征(FAT)大鼠模型及气血亏虚证FAT大鼠模型,观察其下丘脑-垂体-卵巢轴(HPO轴)激素的含量调控,以期为中医药治疗FAT提供一种新的病证结合动物模型。方法采用持续递增负荷运动训练+限制饮食(模型Ⅰ组)、递增力竭游泳+限制饮食(模型Ⅲ组)建立2种FAT大鼠模型,并通过持续递增负荷运动训练+限制饮食+腹腔注射环磷酰胺(模型Ⅱ组)、递增力竭游泳+限制饮食+腹腔注射环磷酰胺(模型Ⅳ组)建立2种气血亏虚证FAT大鼠模型,ELISA法检测大鼠血浆睾酮(T)、孕酮(P)、雌二醇(E_2)、催乳素(PRL)、促黄体素(LH)、卵泡刺激素(FSH)、促性腺激素释放激素(Gn RH)和下丘脑组织匀浆β-内啡肽(β-EP)含量。结果与空白组比较,模型Ⅰ~Ⅳ组大鼠动情期缩短、动情间期延长,β-EP显著增加(P<0.01),Gn RH、P、E_2含量均显著减少(P<0.01),LH含量有减少趋势,而模型Ⅰ、Ⅳ组大鼠PRL显著增加或降低(P<0.01),模型Ⅰ、Ⅱ、Ⅲ组大鼠FSH含量均显著降低(P<0.01),模型Ⅱ、Ⅳ组的大鼠T含量显著减少(P<0.01)。结论持续递增负荷运动训练、力竭游泳合并限制饮食可复制2种相似的FAT大鼠模型,在此基础上腹腔注射环磷酰胺可复制气血亏虚型FAT大鼠模型。
OBJECTIVE: To establish FAT rat model and FAT deficiency rat model of female athlete and to observe the regulation of the hormone in the hypothalamus-pituitary-ovarian axis (HPO axis) in order to provide a method for Chinese medicine treatment of FAT New syndromes combined with animal models. Methods Two kinds of rat models of FAT were established by continuous incremental exercise training + diet restriction (model group Ⅰ), incremental exhaustive swimming + diet restriction (model Ⅲ group), and by continuous incremental exercise training + diet restriction plus intraperitoneal injection Two models of deficiency of qi and blood deficient syndrome (FAT) rats were established by increasing exhaustive swimming, limiting diet and intraperitoneal injection of cyclophosphamide (model group Ⅳ). Plasma testosterone (T) (P), estradiol (E2), prolactin (PRL), LH, FSH, Gn RH and hypothalamic homogenate β- Endorphin (β-EP) content. Results Compared with the blank group, the estrus, the prolongation of estrous period, the prolongation of estrogen, the increase of β-EP (P <0.01) and the decrease of Gn, P and E_2 (P <0.01). The contents of FSH in model group were significantly lower than those in model group (P <0.01) T content in rats decreased significantly (P <0.01). Conclusions Continuous exercise training with increasing load, exhaustive swimming combined with restriction diet can replicate two kinds of similar FAT rat model. On the basis of this, the intraperitoneal injection of cyclophosphamide can copy the blood-deficient FAT rat model.