Using the relativistic quantum molecular dynamics model, we study the influence of multiple scattering on the result of two-pion correlation measurements. The scales of pion spatial distribution are larger at thermal freezeout than at chemical freeze-out. By varying the value of the parameter of cross section from 0 to 90mb, we find that the sizes of pion source measured by two-particle correlation functions are almost independent of the parameter of cross section. However, λ parameters are sensitive to the parameter of cross section.