采用乙酸注射至大鼠胃窦部浆膜下形成慢性胃溃疡模型 ,用游标卡尺测定溃疡指数 ,用 SABC免疫组织化学的方法 ,观察表皮生长因子受体的表达情况并进行图像分析 ,用镀铜镉还原比色法测定血清 NO3- / NO2 - 含量代表血清一氧化氮水平 ,以研究一氧化氮与慢性胃溃疡之间的关系 ,了解在胃溃疡愈合过程中表皮生长因子受体在溃疡周围粘膜的表达 ,探索它们在大鼠慢性胃溃疡的病理生理意义。结果 :1L- Arg组溃疡指数显著低于模型组和 L- NNA组 (P<0 .0 5 ) ,L- NNA组溃疡指数显著高于模型组 (P<0 .0 5 )和 L- Arg组 (P<0 .0 1)。 2模型组和 L- Arg组表皮生长因子受体表达较对照组增加 (积分光密度值及阳性细胞占总面积的百分比均为 P<0 .0 1) ,L - NNA组与对照组和模型组相比无显著性差异 (积分光密度值和阳性细胞占总面积的百分比均为 P>0 .0 5 )。3L - Arg组血清 NO3- / NO2 - 含量显著高于模型组 (P<0 .0 1)及对照组 (P<0 .0 1) ,L - NNA组血清 NO3- / NO2 - 含量显著低于其余 3组。认为正常一氧化氮水平有利于慢性胃溃疡的愈合 ,一氧化氮对表皮生长因子受体的表达具有重要意义 ,这可能是一氧化氮促进慢性胃溃疡愈合的机理之一。 The model of chronic gastric ulcer was established by injecting acetic acid into the submucous of the gastric antrum of rats. The ulcer index was determined by vernier calipers. The expression of epidermal growth factor receptor (EGFR) was observed by SABC immunohistochemistry. Reduction of colorimetric determination of serum NO3- / NO2 - level on behalf of serum nitric oxide levels to study the relationship between nitric oxide and chronic gastric ulcer, to understand the process of gastric ulcer healing of epidermal growth factor receptor in the mucosa around the ulcer Expression and explore their pathophysiological significance in chronic gastric ulcer in rats. Results: The ulcer index in 1L-Arg group was significantly lower than that in model group and L-NNA group (P <0.05). The ulcer index in L-NNA group was significantly higher than that in model group (P <0.05) and L-Arg Group (P <0. 01). The expression of epidermal growth factor receptor in model group and L-Arg group was higher than that in control group (P <0.01), the integral optical density value and percentage of positive cells in the total area were all significantly lower than those in control group and model group There was no significant difference between the two groups (the integral optical density value and the percentage of positive cells in the total area were P> 0.05). The level of NO3 - / NO2 - in serum of 3 - Arg group was significantly higher than that of model group (P <0.01) and control group (P <0. The remaining three groups. It is considered that the normal level of nitric oxide is beneficial to the healing of chronic gastric ulcer. Nitric oxide is of important significance for the expression of epidermal growth factor receptor, which may be one of the mechanisms of nitric oxide in promoting the healing of chronic gastric ulcer.