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Objective: To observe the effects of essential garlic oil (EGO) on vascular cell adhesive molecule 1 (VCAM 1) expression of endothelial cell and monocyte adhesion induced by interleukin 1α (IL 1α). Methods: Human umbilical vein endothelial cells (HUVEC) were isolated by pancreatin digestion method and co cultured with U937 monocyte and IL 1α or EGO+IL 1α respectively. The adhesion rate of monocyte to endothelial cell was measured and mean fluorescent intensity of immuno stained of VCAM 1 was determined by ACAS 570. Results: EGO showed significant inhibition on VCAM 1 expression induced by IL 1α either in simple culture with HUVEC or in co culture with HUVEC+U937, and could obviously down regulate the monocyte endothelial cell adhesion under effect of IL 1α. Conclusion: EGO has the effect of antagonizing against adhesion of monocyte and vascular endothelial cell, it may be due to the inhibition of EGO on adhesive molecular expression on endothelial cell.
Methods: Human umbilical endothelial cells (HUVEC) Isolated by pancreatin digestion method and co cultured with U937 monocyte and IL 1α or EGO+IL 1α respectively. The adhesion rate of monocyte to endothelial cell was measured and mean fluorescent intensity of immunostained of VCAM 1 was determined by ACAS 570. Results: EGO Showed significant inhibition on VCAM 1 expression induced by IL 1α either in simple culture with HUVEC or in co culture with HUVEC+U937, and could obviously down regulate the monocyte endothelial cell adhesion under effect of IL 1α. Conclusion: EGO has the effect of antagonizing Against adhesion of monocyte and vascular endothelial cell, it may be due to the inhibition of EGO on adhesive molecular expression on endothelial cell.