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本文报道了用重量法测定大豆甾醇含量的方法，大豆甾醇含有三种带有３β─ＯＨ基团的甾醇，即菜油甾醇（Ｍ＝４００．６７）、豆ｖｌ甾醇（Ｍ＝４１２．６７）、β─谷甾醇（Ｍ＝４１４．６９），标准样品中该三种甾醇组分含量分别为：１９．５２％、１６．１７％、６４．３１％。大豆甾醇中３β─ＯＨ基团可与洋地黄皂甙（Ｍ＝１２２９．３）反应产生沉淀。１９沉淀相当于大豆甾醇０．２５１ｇ。大豆甾醇平均回收率为１０１．１５±２．３８％，变异系数２．３５％。
This paper reports a method for determining the content of soybean sterol by gravimetric method. Soybean sterol contains three kinds of sterols with 3β-OH group, namely, oleostol (M=400.67) and pea vl sterol (M=412.67). Β-sitosterol (M=414.69). The contents of the three sterol components in the standard sample were: 19.52%, 16.17%, and 64.31%, respectively. The 3β-OH group in soybean sterol can react with digitonin (M=1229.3) to produce precipitate. The 19 precipitate corresponds to 0.251 g of soya sterols. The average recovery rate of soybean sterols was 101.15±2.38% with a coefficient of variation of 2.35%.