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目的:研究人参皂苷Rg1对原代培养正常大鼠大脑皮质神经细胞生物膜的影响。方法:采用细胞培养技术,运用比色法、硫代巴比妥酸法、黄嘌呤氧化酶法(羟胺法)和微量酶标法测定人参皂苷Rg1对细胞内ACP活力、MDA含量、SOD活力及LDH释放量的影响。结果:1mg/ml、2 mg/ml及4 mg/ml人参皂苷Rg1作用30min可不同程度增加正常培养神经细胞的LDH释放量和细胞内SOD活力,降低细胞内ACP活力及MDA含量。结论:1mg/ml、2 mg/ml及4 mg/ml人参皂苷Rg1作用30min对正常培养大脑皮质神经细胞生物膜具有显著影响,其中对溶酶体膜有保护作用,对生物膜的脂质过氧化反应有抑制作用,能减轻自由基对生物膜的攻击及损伤,但对细胞膜可能有一定的损伤作用。
Objective: To study the effects of ginsenoside Rg1 on the biofilm formation of primary cultured cortical neurons in normal rats. Methods: Cell viability, ACP activity, MDA content and SOD activity of ginsenoside Rg1 were assayed by cell culture using colorimetric method, thiobarbituric acid method, xanthine oxidase method (hydroxylamine method) and microplate method Effect of LDH release. Results: The effects of 1 mg / ml, 2 mg / ml and 4 mg / ml ginsenoside Rg1 for 30 min increased the release of LDH and the activity of SOD in normal cultured neurons, and decreased the activity of ACP and the content of MDA. CONCLUSION: The effects of 1 mg / ml, 2 mg / ml and 4 mg / ml ginsenoside Rg1 for 30 min have a significant effect on the culture of neuroblasts in normal cerebral cortex, which has a protective effect on the lysosomal membrane, Oxidation inhibition, can reduce the attack of free radicals on the biofilm and damage, but the cell membrane may have some damage.