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以健康生长的加拿大红枫的叶片、叶柄、顶芽、侧芽为外植体,采用愈伤组织诱导培养方法,通过比较不同的外植体、光照时长、温度、湿度和植物生长调节剂及其配比对加拿大红枫愈伤组织诱导的影响。结果表明:愈伤组织诱导的最适外植体是顶芽;愈伤组织诱导的最适培养基是1/2MS+6-BA 2.0mg/L+NAA 0.1mg/L+GA3 0.1mg/L;生长的最适培养条件为温度(25±2)℃,湿度65%,光照24h/d,光照强度15 00～2 000lx。
The leaves, petioles, terminal buds and lateral buds of healthy Canadian Red Maple were used as explants. Callus induction culture method was used. By comparing different explants, light duration, temperature, humidity and plant growth regulators Effect of Proportion on Callus Induction of Canadian Maple. The results showed that the best explants induced by callus were the terminal buds. The optimal medium for callus induction was 1/2 MS + 6-BA 2.0 mg / L + NAA 0.1 mg / L + GA3 0.1 mg / L The best growth conditions were temperature (25 ± 2) ℃, humidity 65%, light 24h / d, light intensity 1500 ~ 2000lx.