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现有分析鱼藤酮的高效液相色谱方法是针对鱼藤酮的检测而建立的 ,因此它不适合于鱼藤酮类化合物的分析检测。比较鱼藤酮类化合物的UV吸收光谱发现 ,原方法中的检测波长 (2 80nm～ 30 0nm)不适于鱼藤素、毛鱼藤酮及其类似物的检测。通过试验确定适合鱼藤酮类化合物的检测波长为 2 40nm。用CHCl3 MeOH(体积比 9∶1)溶剂提取出的植物中的鱼藤酮类化合物 ,经C18柱进行色谱分离后 ,以MeOH H2 O(体积比 6 6∶34 )为洗脱剂对鱼藤酮类化合物进行等度洗脱。实验结果表明 ,改进的方法可一次性分离检测出鱼藤酮、鱼藤素、毛鱼藤酮及其 12a 羟基 和 6a ,12a 脱氢 类似物 ,各峰分离良好。
The existing method for the analysis of rotenone by HPLC is based on the detection of rotenone and is therefore not suitable for the analysis and detection of rotenone compounds. Comparison of UV absorption spectra of rotenone found that the detection wavelength of the original method (280nm ~ 300nm) is not suitable for the detection of deguelin, dendrone and its analogues. By testing to determine the suitable detection wavelength of rotenone compounds for 2 40nm. The rotenone compounds in plants extracted with CHCl3 MeOH (9: 1 by volume) were separated on a C18 column and eluted with MeOH H2 O (6:34 in volume) for rotenone Isocratic elution. The experimental results show that the improved method can detect rotenone, deguelin, dendrone and its 12a hydroxyl and 6a, 12a dehydrogenation analogs in one step, and the peaks are well separated.