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目的研究双氢青蒿素(DHA)对肝纤维化小鼠Ⅰ型胶原表达的影响。方法将24只C57BL/6野生型雄性小鼠分为对照组、模型组、观察组,每组8只。应用四氯化碳(CCl4)腹腔内注射诱导小鼠肝纤维化模型,在腹腔内注射CCl4 8次后,按20μg/g体质量的DHA原料药给予观察组小鼠灌胃,每天1次。等容积溶剂给予对照组、模型组灌胃。各组小鼠在注射12次后24h取肝组织。组织切片采用苏木精—伊红染色(H&E)染色、Ⅰ型胶原免疫组织化学染色评价肝纤维化模型建立是否成功,并对比研究模型组和观察组的肝组织Ⅰ型胶原表达的变化。结果模型组及观察组肝组织病理证实肝纤维化模型复制成功,观察组Ⅰ型胶原染色面积较对照组明显下降,差异有统计学意义(P<0.05)。结论双氢青蒿素能够降低CCl4诱导的小鼠肝组织Ⅰ型胶原沉积程度。
Objective To study the effect of dihydroartemisinin (DHA) on the expression of type Ⅰ collagen in mice with hepatic fibrosis. Methods Twenty-four C57BL / 6 wild-type male mice were divided into control group, model group and observation group, with 8 in each group. The model of hepatic fibrosis induced by intraperitoneal injection of carbon tetrachloride (CCl4) was induced in mice. After intraperitoneal injection of CCl4 for 8 times, mice in the observation group were given intragastrically with DHA API at a dose of 20 μg / g body weight once daily. The volume of solvent given to the control group, model group gavage. The mice in each group were taken liver tissue 24 hours after injection for 12 times. Tissue sections were stained with hematoxylin-eosin (H & E) staining and collagen type Ⅰ immunohistochemistry to evaluate whether the establishment of hepatic fibrosis model was successful. The changes of collagen type Ⅰ expression in the model group and the observation group were compared. Results The hepatic fibrosis model was successfully duplicated in model group and observation group. The staining area of type Ⅰ collagen in observation group was significantly lower than that in control group (P <0.05). Conclusion Dihydroartemisinin can reduce the level of collagen Ⅰ in CCl4-induced liver tissue of mice.