A molecularly imprinted polymer (MIP), with special molecule recognition properties of ciprofloxacin (CIP), was prepared by thermal polymerization in which ciprofloxacin acted as template molecule, α-methacrylic acid (MAA) acted as functional monomer and trimethy-lolpropane trimethylacrylate (TRIM) acted as crosslin-ker. The optimized ratio was determined to be n(CIP): n (MMA):n(TRIM) = 1:6:16 by investigation of the effects of different concentrations of functional monomer and the crosslinker on the MIP’s recognition properties. Equilibrium binding experiment was used to investigate the adsorption dynamics, the binding ability to template molecule and the substrate selectivity. Scatchard analysis was used to study the MIP’s binding characteristic to tem-plate molecule. The results indicated that MIP has higher adsorption ability and selectivity. The equilibrium distri-bution coefficient KD was 41.64 and the separation factor α was 1.62. Scatchard analysis showed that two different kinds of binding sites were produced in the polymer matrix and their dissociation constants were calculated to be Kd1= 5.249×10-5 mol·L-1, Kd2=2.237×10-3mol·L-1.