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目的研究白细胞分化抗原14(CD14)在急性脑出血应激性肝损害内毒素信号转导通路中的作用以及星蒌承气汤的靶向调控机制。方法采用大脑注射Ⅶ型胶原酶法建立SD大鼠急性脑出血模型,随机分为正常组、脑出血组、脑出血痰热腑实证组、星蒌承气汤组,造模后24、48、72 h实时荧光定量PCR观察肝组织CD14 mRNA表达,并观察肝组织HE染色结果。结果脑出血急性期肝组织CD14 mRNA表达升高,星蒌承气汤能降低急性脑出血痰热腑实证肝组织CD14 mRNA表达,并能明显减轻肝组织病理损伤。结论 CD14在介导内毒素性肝损伤中起重要作用,星蒌承气汤对脑出血急性期痰热腑实证肝脏具有一定的保护作用。
Objective To investigate the role of leukocyte differentiation antigen 14 (CD14) in the endotoxin signal transduction pathway of acute hepatic injury induced by acute intracerebral hemorrhage and the regulatory mechanism of Xinglou Chengqi Decoction. Methods Acute cerebral hemorrhage model of SD rats was established by intracerebral injection of collagenase type Ⅶ. The rats were randomly divided into four groups: normal group, cerebral hemorrhage group, positive experimental group of phlegm and phlegm of cerebral hemorrhage, The expression of CD14 mRNA in liver tissue was observed by real-time fluorescence quantitative PCR at 72 h and the HE staining results of liver tissue were observed. Results The expression of CD14 mRNA in acute liver injury in patients with cerebral hemorrhage increased. Xinglou Chengqi Decoction could reduce the expression of CD14 mRNA in the liver tissue of experimental rats with acute cerebral hemorrhage and reduce the pathological damage of liver tissue. Conclusion CD14 plays an important role in mediating endotoxin-induced liver injury. Xinglou Chengqi Decoction has a certain protective effect on the experimental liver of phlegm and heatfueling in acute stage of cerebral hemorrhage.