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前列腺素(PG)D_2是天然存在的有着各种药理学作用的廿碳化合物。在皮肤,尽管PGD_2在表皮和真皮中是来自花生四烯酸的主要环氧合酶产物之一,但其在体内的功能还不清楚。本文通过研究中波紫外线(UVB)照射后大鼠皮肤PGD合成酶活性的变化来阐明PGD_2在UVB诱导炎症中的作用。 200～250g雄性Wistar大鼠,UVB照射量为500mJ/cm~2,照射前48小时剃毛,分别于照射前及照射后3、6、12和24小时取材,按Raineri等人方法进行表皮与真皮分离,然后将组织制备成匀浆,在4℃经100 000g离心1小时,取其上清液,在谷胱甘肽(GSH)存在下,用特异的放射免疫测定法进
Prostaglandin (PG) D 2 is a naturally occuring twenty-carbon compound with various pharmacological effects. On the skin, although PGD2 is one of the major cyclooxygenase products from arachidonic acid in the epidermis and dermis, its function in vivo remains unclear. In this paper, we studied the effect of PGD_2 on UVB-induced inflammation by studying the changes of PGD synthase activity in rat skin after UVB irradiation. 200 ~ 250g male Wistar rats were shaved for 48 hours before UVB irradiation at a UVB dose of 500mJ / cm ~ 2, and were harvested before and 3,6,12 and 24 hours after irradiation, respectively. According to Raineri et al. The dermis was separated and the tissue was then homogenized and centrifuged at 100 000 g for 1 hour at 4 ° C. The supernatant was taken and subjected to specific radioimmunoassay in the presence of glutathione (GSH)