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目的研究29例变异型Ph易位慢性粒细胞白血病(CML)患者衍生9号染色体[der(9)]部分序列的缺失情况。方法患者骨髓标本采用直接法和短期培养法制备染色体,采用R显带技术进行核型分析。运用BCR-ABL双色双融合DNA探针对骨髓间期细胞进行荧光原位杂交(FISH)技术检测der(9)染色体部分序列的缺失。结果29例变异型Ph易位CML患者中,4例伴有缺失,4例Ph+中期和间期细胞均伴有缺失。结论在变异性Ph易位(CML)病患者中der(9)缺失的发生率13.7%,该缺失是不良预后指标。
Objective To investigate the deletion of partial sequence of der (9) derived from chromosome 9 in 29 patients with variant Ph translocation chronic myeloid leukemia (CML). Methods The bone marrow samples of patients were prepared by direct method and short-term culture method, and the karyotype analysis was performed by R-banding technique. The BCR-ABL double-stranded double-fusion DNA probe was used to detect the deletion of part of der (9) chromosome sequence by fluorescence in situ hybridization (FISH). Results Among the 29 patients with variant Ph translocation CML, 4 patients had deletion and 4 patients with Ph + metaphase and interphase cells were all missing. Conclusions The incidence of der (9) deletion in patients with variant Ph translocation (CML) is 13.7%, a marker of poor prognosis.