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观察成骨肉瘤患者辅助性Ｔ淋巴亚群中Ｔｈ１／Ｔｈ２亚群的变化，为细胞因子免疫治疗提供依据。方法应用放射免疫法以及酶联免疫吸附法检测成骨肉瘤患者血清中ＩＬ－２、ＩＬ－４、ＩＬ－６、ＴＮＦ－α的含量，检测外周血单个核细胞培养上清中ＩＬ－２、ＩＬ－４含量。结果成骨肉瘤血清中及培养上清中ＩＬ－２减少，而ＩＬ－４、ＩＬ－６、ＴＮＦ－α含量增加。结论成骨肉瘤患者中存在有Ｔｈ１／Ｔｈ２平衡失调，其中Ｔｈ１亚群功能抑制，Ｔｈ２亚群功能亢进，它们与肿瘤在宿主体内生长密切相关。
To observe the changes of Th1/Th2 subsets in T lymphocyte subsets of osteosarcoma patients and provide evidence for cytokine immunotherapy. Methods The levels of IL-2, IL-4, IL-6 and TNF-α in serum of patients with osteosarcoma were detected by radioimmunoassay and enzyme-linked immunosorbent assay. IL-2 was detected in culture supernatant of peripheral blood mononuclear cells. IL-4 content. Results IL-2 was decreased in the serum and culture supernatant of osteosarcoma, but the levels of IL-4, IL-6 and TNF-α increased. Conclusion Th1/Th2 balance disorder exists in osteosarcoma patients, in which Th1 subgroup function is inhibited and Th2 subgroup is hyperfunctioning. They are closely related to tumor growth in the host.