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目的探讨屋尘螨过敏原质粒 DNA 疫苗对屋尘螨致敏/激发小鼠气道变应性炎症的作用。方法 40只小鼠分为健康对照组、屋尘螨致敏/激发模型组(用屋尘螨提取液致敏/激发)、Derp1治疗组(用屋尘螨提取液致敏/激发,用编码有屋尘螨过敏原 Der p1的真核表达质粒肌肉注射后再次激发)、Der p2治疗组(用屋尘螨提取液致敏/激发,用编码有屋尘螨过敏原 Der p2的真核表达质粒肌肉注射后再次激发)、Der p1联合 Der p2治疗组(用屋尘螨提取液致敏/激发,用编码有屋尘螨过敏原 Der p1和 Der p2的真核表达质粒肌肉注射后再次激发)5组,每组8只。检测5组小鼠支气管肺泡灌洗液(BALF)中细胞计数和分类。取5组小鼠肺组织行病理检查。测 BALF、血清中 IL-4、IL-5、IFNγ水平及 BALF 中 IgE、IgG1水平。结果屋尘螨致敏/激发模型组小鼠肺组织可见明显炎性细胞浸润;BALF 中炎性细胞特别是嗜酸性粒细胞计数较健康对照组明显升高(P<0.01),IL-4、IL-5、IgE、IgG1水平较健康对照组明显升高(P<0.001),IFNγ水平与健康对照组比较差异无统计学意义(P<0.01)。经 DNA 疫苗治疗后,小鼠肺部炎性细胞浸润显著减少;BALF 中炎性细胞计数及 IL-4、IL-5、IgE、IgG1水平显著下降,IFNγ水平无显著变化,IL-10水平显著升高。Der p1联合 Der p2治疗组各项指标的改善情况优于 Der p1治疗组和 Der p2治疗组。结论屋尘螨过敏原质粒 DNA 疫苗可有效抑制屋尘螨致敏/激发小鼠气道变应性炎症,且两种疫苗联合治疗优于单用一种疫苗治疗。
Objective To investigate the effect of house dust mite allergen DNA vaccine on allergic airway inflammation in mice induced by house dust mites. Methods Forty mice were divided into healthy control group, house dust mite sensitization / challenge model group (sensitized / challenged with house dust mite extract), Derp1 treatment group (sensitized / challenged with house dust mite extract, Eukaryotic expression plasmid Der p 1 with house dust mite allergen re-challenged after intramuscular injection), Der p2 treated group (sensitized / challenged with house dust mite extract, eukaryotic expression encoding Der p2 of House dust mite allergen Plasmids were re-challenged after intramuscular injection), Der p 1 combined with Der p 2 treatment group (sensitized / challenged with house dust mite extracts, re-challenged with intramuscular injection of eukaryotic expression plasmids encoding house dust mite allergens Der p 1 and Der p 2 ) 5 groups of 8. The cell counts and classification in bronchoalveolar lavage fluid (BALF) of 5 groups of mice were detected. Take 5 groups of mice lung tissue pathological examination. BALF, serum IL-4, IL-5, IFNγ levels and BALF IgE, IgG1 levels were measured. Results The inflammatory cells, especially the eosinophils in BALF were significantly increased in the lung tissue of mice induced by house dust mite (P <0.01), while the levels of IL-4, The levels of IL-5, IgE and IgG1 were significantly higher than those of the healthy controls (P <0.001). There was no significant difference in IFNγ levels between the two groups (P <0.01). Inflammatory cell infiltration in lungs of mice was significantly reduced after treatment with DNA vaccine. Inflammatory cytokines and IL-4, IL-5, IgE and IgG1 levels in BALF were significantly decreased, but there was no significant change in IFNγ level and IL-10 level Rise. Der p1 with Der p2 treatment group improved better than the Der p1 treatment group and Der p2 treatment group. Conclusions The DNA vaccine against house dust mite allergen can effectively inhibit the airway allergic inflammation induced by house dust mite and the combination of the two vaccines is superior to the vaccine alone.