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Two novel steroidal saponins (timosaponin BⅢ-a,timosaponin BⅢ-b) together with a known steroidal saponin (timosaponin BⅡ-b) were prepared by the dilute acid hydrolysis of timosaponin BⅢ Their structures were elucidated by means of 1D,2D NMR and TOF-MS spectral analysis.