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目的探讨葡萄糖-6-磷酸异构酶(GPI)抗原在类风湿关节炎(RA)临床诊断和病情评估中的价值。方法采用ELISA方法检测108例RA患者、70例非RA患者及72例健康人血清中的GPI抗原表达水平,同时检测类风湿因子(RF)。结果 RA患者血清RF和GPI抗原阳性表达率明显高于非RA患者组和正常对照组(P﹤0.05),且GPI抗原阳性表达率更高于RF阳性表达率;GPI抗原检测的敏感性(79.6%)和特异性(96.2%)均高于RF(68.5%,85.2%)(P﹤0.05);活动性RA患者GPI抗原的表达水平明显高于非活动性RA患者(P﹤0.05);不同的X线分期GPI抗原水平差异有统计学意义(P﹤0.05)。结论 GPI抗原检测对RA的诊断具有良好的敏感性和特异性,并与RA的活动性及病程进展相关,可应用于RA的临床诊断和病情评估。
Objective To investigate the value of glucose-6-phosphate isomerase (GPI) antigen in the clinical diagnosis and assessment of rheumatoid arthritis (RA). Methods Serum levels of GPI in 108 RA patients, 70 non-RA patients and 72 healthy controls were detected by ELISA. Rheumatoid factor (RF) was also detected. Results The positive rate of RF and GPI antigen in RA patients was significantly higher than that in non-RA patients and normal controls (P <0.05), and the positive rate of GPI antigen was higher than that of RF. The sensitivity of GPI antigen test (79.6 %) And specificity (96.2%) were higher than that of RF (68.5%, 85.2%) (P <0.05). The GPI antigen expression in active RA patients was significantly higher than that in inactive RA patients (P <0.05) There was significant difference in the GPI antigen level between the two groups (P <0.05). Conclusion The detection of GPI antigen has good sensitivity and specificity for the diagnosis of RA. It is related to the activity and progression of RA and can be used in the clinical diagnosis and assessment of RA.