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[目的]检测温室土壤中有机提取物的遗传毒性作用。[方法]以小鼠为实验动物,共分5组:阴性对照组(二甲亚砜)、阳性对照组(环磷酰胺)及土壤有机提取物低、中、高剂量组,染毒剂量分别为5、15和30g土壤干重/(kg小鼠体重.d),染毒方式为每日灌胃1次,连续染毒4周。用胞质分裂阻滞微核细胞试验和彗星试验分别检测有机提取物所致小鼠外周血淋巴细胞的染色体损伤作用和外周血细胞的DNA损伤作用。[结果]有机提取物剂量与微核率具有较明显的剂量-效应关系,显著高于阴性对照组;随着有机提取物作用剂量的增加,彗星尾长、尾部DNA含量和Olive尾矩显著升高。[结论]温室土壤中有机提取物可以诱使小鼠发生染色体和DNA损伤。
[Objective] The research aimed to detect the genotoxicity of organic extracts in greenhouse soil. [Methods] The mice were divided into 5 groups: negative control group (dimethyl sulfoxide), positive control group (cyclophosphamide) and soil organic extracts low, medium and high dose groups, respectively, dose 5, 15 and 30g soil dry weight / (kg mouse body weight .d), the method of administration was daily gavage 1, continuous exposure for 4 weeks. Cytokinesis of cytokinesis and micronuclei in comet assay were used to detect the chromosomal damage of peripheral blood lymphocytes and the DNA damage of peripheral blood cells induced by organic extracts. [Result] The dose of organic extract had a more obvious dose-effect relationship with micronucleus rate, which was significantly higher than that of the negative control group. With the increase of the dose of organic extract, the tail DNA content and Olive tail moment of comet significantly increased high. [Conclusion] Organic extracts in greenhouse soil can induce chromosomal and DNA damage in mice.