论文部分内容阅读
目的 实验观察山莨菪碱对细菌脂多糖 (L PS)结合血管内皮细胞膜及诱导 NO生成的影响 .方法 体外分离培养牛主动脉血管内皮细胞 ,用免疫组化显色反应显示细菌脂多糖与血管内皮细胞的结合 ,并通过图像分析对结果进行半定量 ;用高压液相色谱分析细胞培养液中 NO3-的含量 ,间接反应 NO的生成水平 .结果 细胞分组处理 ,间接法显色后图像分析 ,结果表明山莨菪碱组的平均灰度值明显低于 L PS组 (P<0 .0 5 ) ;并且对细胞培养液中 NO3- 的检测显示山莨菪碱组的浓度明显低于 L PS组 (P<0 .0 5 ) .结论 山莨菪碱能够抑制 L PS结合血管内皮细胞 ,并且能够抑制 L PS对 NO合成的诱导作用
Objective To observe the effect of Anisodamine on bacterial membrane lipopolysaccharide (LPS) binding to vascular endothelial cell membrane and the induction of NO production.Methods Bovine aortic endothelial cells (VECs) were isolated and cultured in vitro and the expressions of lipopolysaccharide and vascular endothelium Cell binding, and the results were semi-quantitatively analyzed by image analysis.High-pressure liquid chromatography was used to analyze the content of NO3- in cell culture medium and indirect reaction of NO production.Results Cell grouping, indirect colorimetric image analysis, the results The results showed that the average gray value of anisodamine group was significantly lower than that of L PS group (P <0.05), and NO3- in cell culture medium showed that the concentration of anisodamine group was significantly lower than that of LPS group (P <0.05) Conclusion Anisodamine can inhibit LPS-binding to vascular endothelial cells and inhibit the induction of NO production by LPS