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应用［１４Ｃ］－青霉素对５０株铜绿假单胞菌进行了青霉素结合蛋白亲和力及与膜通透性、β-内酰胺酶、药物敏感性间关系的研究，并对液闪同位素计数法测定青霉素蛋白结合率进行了尝试。结果表明：①亚胺培能对铜绿假单胞菌的致死靶位蛋白为ＰＢＰｓ２。②铜绿假单胞菌临床分离株对亚胺培能耐药率达１４％；所有敏感株的膜通透率（３５．０％）与ＰＢＰｓ结合率（４６．５８％）均高于耐药株（２３．６１％、３４．５０％，Ｐ＜０．０５）。③耐药株中高产酶株（ ～ ）可达２８．５９％远高于敏感株１３．９５％（Ｐ＜０．０１），其β－内酰胺酶产酶量越高则膜通透率及ＰＢＰｓ结合率越低。④无论膜通透率是０还是１００％，ＰＢＰｓ结合率无明显改变。结果提示：耐碳青烯类抗生素的铜绿假单胞菌，其耐药机制是多方面的，主要与细菌膜通透性改变及β-内酰胺酶水解有关，而与ＰＢＰｓ无关。
The application of [14C] -penicillin to 50 strains of Pseudomonas aeruginosa was studied for the affinity of penicillin-binding protein and its relationship with membrane permeability, β-lactamase and drug sensitivity, and the determination of penicillin by liquid-flash isotope counting Protein binding rate has been tried. The results showed that: (1) Imipenem caused the lethal target protein of Pseudomonas aeruginosa PBPs2. ② The susceptibilities of Pseudomonas aeruginosa isolates to imipenem were 14%; the membrane permeability (35.0%) and PBPs binding rate (46.58%) of all the sensitive strains were higher than that of drug-resistant Strains (23.61%, 34.50%, P <0.05). (3) The resistant strains of high yield enzyme (~) up to 28.59% higher than the sensitive strain 13.95% (P <0.01), the higher β-lactamase enzyme production of membrane permeability And the lower the binding rate of PBPs. ④ No matter the membrane permeability is 0 or 100%, the binding rate of PBPs has no obvious change. The results suggest that the mechanism of resistance to carbapenem-resistant Pseudomonas aeruginosa is multifaceted, mainly related to changes in bacterial membrane permeability and β-lactamase hydrolysis, but not to PBPs.