采用高效液相色谱法建立杜仲果仁中桃叶珊瑚苷的含量测定方法,探讨了不同提取溶剂和提取方法对测定结果的影响。结果表明:以甲醇做提取溶剂,采用冷浸法多次提取,效果最佳。此法样品处理简单,线性范围宽,重现性好,精密度高,效果令人满意,对于杜仲籽的质量控制具有一定的指导意义。 High performance liquid chromatography was used to determine the content of aucubin in Eucommia ulmoides kernels and the effects of different extraction solvents and extraction methods on the determination results were discussed. The results showed that the extraction solvent was methanol, extracted by cold soaking method for several times, the effect was the best. The method has the advantages of simple processing, wide linear range, good reproducibility, high precision and satisfactory effect, and has certain guiding significance for the quality control of Eucommia seed.