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目的:探讨加味茵陈蒿汤对高脂饮食小鼠肝组织超氧化物歧化酶(SOD)活力及丙二醛(MDA)含量的影响。方法:60只雌性昆明种小鼠经高脂饲料喂养6周后,随机分为模型对照组,0.5、1、2倍等效临床剂量加味茵陈蒿汤组及对照药山楂精降脂片组;另设12只为普食对照组。各组分别灌胃给药4周后检测肝组织匀浆上清SOD活力和MDA含量。结果:高脂饮食可导致肝组织SOD活力降低及MDA含量升高;加味茵陈蒿汤及山楂精降脂片均可使肝组织SOD活力提高(P<0.01),并恢复至正常水平;与模型对照组比较,加味茵陈蒿汤低剂量组可降低肝组织MDA含量(P<0.05),其余各组对MDA含量的改变无统计学差异。结论加味茵陈蒿汤能有效改善高脂饮食小鼠的抗氧化能力,对该方药理学机制的进一步研究可为该方防治高脂血症及其并发症提供新的思路和依据。
Objective: To investigate the effect of Yinchenhao decoction on the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in the liver tissue of high fat diet mice. Methods: Sixty female Kunming mice were fed with high-fat diet for 6 weeks and were randomly divided into model control group, 0.5,1,2-fold equivalent clinical dose of Yinchenhao Decoction group and control group hawthorn fine lipid lowering group ; Another 12 for the general diet control group. The rats in each group were given gavage for 4 weeks, then the superoxide dismutase (SOD) activity and MDA content in liver homogenate were detected. Results: High-fat diet could reduce the activity of SOD and increase the content of MDA in liver tissue. The addition of Yinchenhao decoction and hawthorn serum lipid-lowering tablets could increase the activity of SOD in liver tissue (P <0.01) and return to the normal level. Compared with the model control group, the low-dose Yinchenhao decoction could reduce the content of MDA in the liver tissue (P <0.05), while the other groups had no significant difference in the content of MDA. Conclusion Modified Yinchenhao decoction can effectively improve the anti-oxidation ability of mice in high-fat diet. Further study on this pharmacological mechanism may provide new ideas and basis for the prevention and treatment of hyperlipidemia and its complications.