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目的:探讨原发性肝细胞癌(hepatocellular carcinoma,HCC)组织中免疫细胞的浸润及分布情况。方法:采用免疫组化S-P法,检测采集自福建省肿瘤医院30例HCC癌组织、癌周组织(距肿瘤边缘<2cm)、非癌肝组织(距肿瘤边缘>5cm)石蜡标本中CD3、CD4、CD8、FoxP3、CD20、CD56、CD68的表达。结果:免疫组织化学结果显示,HCC患者CD3+、CD4+、FoxP3+细胞数在癌周组织中最高,癌组织中其次,非癌肝组织中最低(P<0.05);CD8+、CD56+、CD68+细胞数在癌周组织中最高,非癌肝组织其次,癌组织中最低(P<0.05);CD20+细胞数在肝癌组织、癌周组织、非癌肝组织中无明显差异(P>0.05)。肝癌组织中CD3+、CD4+、CD8+、CD56+、CD68+细胞主要分布于癌巢中的间质区,FoxP3+细胞呈散在分布。结论:肝癌组织局部微环境中,杀伤性免疫细胞减少,抑制性免疫细胞增加,从而导致局部免疫抑制状态。
Objective: To investigate the infiltration and distribution of immune cells in primary hepatocellular carcinoma (HCC). Methods: Immunohistochemical SP method was used to detect the expression of CD3, CD4 in the specimens from 30 cases of HCC, adjacent non-cancerous liver tissues (> 5 cm away from the tumor margin) , CD8, FoxP3, CD20, CD56, CD68. Results: The results of immunohistochemistry showed that the number of CD3 +, CD4 +, FoxP3 + cells in HCC patients was the highest among all the cancerous tissues and the lowest in non-cancerous tissues (P <0.05). The number of CD8 +, CD56 + and CD68 + The highest in non-cancerous tissues and the second in cancerous tissues were the lowest (P <0.05). There was no significant difference in the number of CD20 + cells between hepatocellular carcinoma, pericarcinoma and non-cancerous liver tissues (P> 0.05). The number of CD3 +, CD4 +, CD8 +, CD56 + and CD68 + cells in HCC tissues mainly distributed in the interstitial region of cancer nests. FoxP3 + cells were scattered. Conclusion: In the local microenvironment of HCC tissue, the number of killer immune cells decreases and the number of inhibitory immune cells increases, leading to a local immunosuppressive state.