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目的：研究醋酸甲羟孕酮（ＭＰＡ）的电极反应机理，拟定了测定ＭＰＡ的单扫描示波极谱法。方法：用单扫描示波极谱法、快速扫描循环伏安法、恒电位电解法、紫外分光光度法等多种技术进行研究。结果：在醋酸盐缓冲溶液中，ＭＰＡ的Ｃ＝Ｃ双键首先发生单电子单质子还原，产生了中间体自由基ＨＭＰＡ·。随后ＨＭＰＡ·可以单电子单质子方式进一步还原，形成产物Ｈ２ＭＰＡ；同时也可以与中性ＭＰＡ分子形成二聚体自由基ＨＭＰＡ·ＭＰＡ·。在０２ｍｏｌ·Ｌ－１ＨＡｃ—ＮａＡｃ缓冲液中，ＭＰＡ还原波的二阶导数峰电流ｉＰ″与其浓度ＣＭＰＡ在２０×１０－６～６０×１０－５ｍｏｌ·Ｌ－１范围内成线性关系，相关系数γ＝０９９８。结论：证实ＭＰＡ的还原过程有中间体自由基参与。
Objective: To study the electrode reaction mechanism of medroxyprogesterone acetate (MPA) and to develop a single-scan oscillopolarographic method for the determination of MPA. Methods: Various techniques such as single-sweep oscillopolarography, rapid scanning cyclic voltammetry, potentiostatic electrolysis and UV spectrophotometry were studied. Results: In acetic acid buffer solution, the single C = C double bond of MPA first reduced to a single electron proton, resulting in the intermediate radical HMPA ·. HMPA · can then be further reduced by single-electron single-proton to form the product H2MPA; at the same time, HMPA · MPA · can also form dimer free radical with neutral MPA molecules. In 02mol·L-1HAc-NaAc buffer, the second derivative peak current iP of MPA reduction wave and the concentration of CMPA in the range of 2.0 × 10-6 ~ 6.0 × 10-5mol·L-1 Linear relationship, the correlation coefficient γ = 0998.Conclusion: The MPA reduction process confirmed the intermediate free radical participation.